A simple and selective LC method is described for the determination of Netupitant and Palonosetron in tablet dosageforms. Chromatographic separation was achieved on a C18 column using mobile phase consisting of a 55 volumes ofmixed phosphate buffer and 45 volumes of acetonitrile were prepared. with detection of 253nm. Linearity wasobserved in the range 25-125 μg/ml for Netupitant(r2 =0.995) and 50-150 μg /ml for Palonosetron(r2 =0.999) for theamount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposedmethods were validated. The accuracy of the methods was assessed by recovery studies at three different levels.Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives.The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. Allstatistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.Key Words: Netupitant, Palonosetron, LC method

A simple and selective LC method is described for the determination of Aceclofenac and Thiocolchicoside in tabletdosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixtureof 0.1M ammonium acetate:Methanol with detection of 248 nm. Linearity was observed for Aceclofenac r2 =0.994)and for Thiocolcicoside(r2 =0.995) for the amount of drugs estimated by the proposed methods was in goodagreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed byrecovery studies at three different levels. Recovery experiments indicated the absence of interference fromcommonly encountered pharmaceutical additives. The method was found to be precise as indicated by therepeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be usedfor routine analysis of pharmaceutical dosage form.Key Words: Aceclofenac, Thiocolchicoside, tablet dosage forms

ABSTRACTA simple and selective LC method is described for the determination of Gemcitabine and Capecitabine in tabletdosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixtureof 80 volumes of methanol and 20 volumes of water with detection of 240 nm. Linearity was observed in the range 6-14 μg /ml for Gemcitabine (r2 =0.998) and 6-14 μg /ml for Capecitabine (r2 =0.996) for the amount of drugsestimated by the proposed methods was in good agreement with the label claim. The proposed methods werevalidated. The accuracy of the methods was assessed by recovery studies at three different levels. Recoveryexperiments indicated the absence of interference from commonly encountered pharmaceutical additives. Themethod was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statisticaldata proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.Key Words: Gemcitabine, Capecitabine, pharmaceutical dosage form

A simple and selective LC method is described for the determination of Donepezil and Memantine dosage forms.Chromatographic separation was achieved on a C18 column using mobile phase consisting of a mixture of Methanol:Acetonitrile: Water (55: 25: 20 v/v/v), with detection of 277nm. Linearity was observed in the range 20-60 μg /ml forDonepezil (r2 =0.998) and 40-120μg/ml for Memantine(r2 =0.999) for the amount of drugs estimated by the proposedmethods was in good agreement with the label claim.The proposed methods were validated. The accuracy of themethods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence ofinterference from commonly encountered pharmaceutical additives. The method was found to be precise as indicatedby the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and canbe used for routine analysis of pharmaceutical dosage form.Key Words: Donepezil, Memantine, Chromatographic separation

A simple and selective LC method is described for the determination of Sitagliptin and Simvastatin in tablet dosageforms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of 40volumesof Mixed buffer, 40 volumes methanol and 20 volumes of Acetonitrile with detection of 241 nm. Linearitywas observed in the range 60-140μg /ml for Sitagliptin(r2 =0.997) and 61-155μg /ml for Simvastatin (r2 =0.997) forthe amount of drugs estimated by the proposed methods was in good agreement with the label claim. From the aboveexperimental results and parameters, it was concluded that, this newly developed method for the simultaneousestimation Sitagliptin and Simvastatin was found to be simple, precise, accurate and high resolution and shorterretention time makes this method more acceptable and cost effective and it can be effectively applied for routineanalysis in research institutions, quality control department in meant in industries, approved testing laboratoriesstudies in near future.Key Words: Sitagliptin, Simvastatin, tablet dosage forms, LC method

Different polymeric Patches containing Haloperidol were prepared and evaluated for physicochemical, in vitrodrug release and Kinetic studies. The IR spectral analysis of Haloperidol showed that the principal peaks and for themixture of Haloperidol with different polymers additional to the principal peaks, some additional peaks wereobserved with physical mixtures, which could be due to the presence of polymers. The presence of all thecharacteristic bands due to functional groups in polymer mixtures suggests that there is no interaction between thedrug and polymers used in the present study. The prepared transdermal patches were evaluated for theirphysiochemical characteristics such as physical appearance, weight uniformity, thickness, folding endurance;moisture content, drug content were suitable. Transdermal patches with Xanthum gum showed better release thanpatches with Guar Gum. The release rate was increased with an increase in Xanthum gum content. The releasekinetics of the optimized formulations followed Higuchi and release mechanism was Non-fickian diffusion ratecontrolled mechanism.Key Words: Haloperidol, Transdermal patches, Xanthum gum

The method described by Touitou et al., (2000) was employed with little modification for the preparation of variousethosomal formulations containing different concentration of ethanol (20 % to 40 %) with sonication . Thetechniques used were simple and reproducible. The prepared ethosomes were spherical and discrete in shape.However ethosomes prepared by sonication method were more uniform and small in size which is essential for skinpenetration. While comparing the entrapment efficiency, ethosomes containing 40% w/w ethanol and prepared bysoncation showed highest value respect to all other formulation; so it is concluded ethosomal prepared by sonicationand containing 40 % w/w ethanol as the best formulation considering all other aspectsIncrease inthepolymerconcentrationledto increase in,%Drugentrapment efficiency, Particle size. The invitro drugreleasedecreased with increase in thepolymer and copolymerconcentration.Among all formulations LE6 showsMaximum drug release upto 24hrs and shows maximum drug release.Analysis ofdrug release mechanismshowedthatthedrug release fromthe formulations followed the Non fickian diffusion mechanism and follows 1storderkinectics.Based on the results of evaluation tests formulation code LE6 was concluded as best formulation.Key Words: Ethosomes, Sonication, Transdermal,Entrapment, Stability

Matrix tablets were compressed without any problem and do not require any change in ratio of excipients informulation. Results of the present study demonstrated that natural polymers could be successfully employed forformulating sustained-release matrix tablets ofNateglinide. The release of drug depends not only on the nature ofmatrix but also upon the concentration of polymer. As the percentage of polymer increased, the rate of releasedecreased. The formulation F6 was optimized because drug release was sustained up to 12hrs.Key Words: Nateglinide, Matrix tablets, optimized drug release

Posaconazole, a broad-spectrum triazole antifungal agent, is approved for the prevention of invasive as per gillosisand candidiasis in addition to the treatment of oropharyngeal candidiasis. There is evidence of efficacy in thetreatment and prevention of rarer, more difficult-to-treat fungal infections. To alleviate this problem, vesicular drugdelivery system transfersomes is formulated to deliver Posaconazole across skin and target drug to synovium orspecific tissues which in turn increase drug efficacy with minimum extra synovial toxicity.The present researchwork involves formulation and in-vitro evaluation of Posaconazole transferosomal gel to reduce dosing frequency.The FTIR spectra revealed that there was no interaction between the drug and excipients. Transfersomeformulations were prepared by thin film hydration technique and were incorporated into 1.5% carbapol gel. TheFormulation PF7 containing Lecithin: Tween-80 in ratio 70:30 (%w/w) has higher entrapment efficiency andmaximum drug release. In-vitro skin permeation study studies showed that,transfersome gels were found to increasethe skin permeation and deposition showing a sustain effect. Stability studies performed for optimizedtransferosome gel formulations indicates that prepared transferosomes have more stability at lower temperature.Based on the above data, it was confirmed that prepared Posaconazole, transpersonal gels can be considered as oneof the promising approaches to reduce the dosing frequency and to maintain drug concentration at the desired sitefor longer time.Key Words: Posaconazole, transferosome gel formulations