ABSTRACT
The estimation of Lamivudine and Raltegravir was done by RP-HPLC. The assay of Lamivudine and Raltegravir was performed with tablets and the % assay was found to be 100.48 and 98.84 which shows that the method is useful for routine analysis. The linearity of Lamivudine and Raltegravir was found to be linear with a correlation coefficient of 0.998 and 0.999, which shows that the method is capable of producing good sensitivity. The acceptance criteria of precision is RSD should be not more than 2.0% and the method show precision 1.31 and 0.96 for Lamivudine and Raltegravir which shows that the method is precise. The validation of developed method shows that the accuracy is well within the limit, which shows that the method is capable of showing good accuracy and reproducibility.
Key Words: Lamivudine, Raltegravir, RP-HPLC

ABSTRACT
A new method was established for simultaneous estimation of Empagliflozin and Linagliptin by RP-HPLC. The
chromatographic conditions were successfully developed for the separation of Empagliflozin and Linagliptin by
using Agilent C18 column (4.6×150mm)5μ, flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) methanol:
phosphate buffer(KH2PO4and K2HPO4) phosphate pH 3 ( pH was adjusted with orthophosphoricacid),detection
wavelength was 254 nm.
The % purity of Empagliflozin and Linagliptin was found to be 99.87% and 100.27% respectively. The linearity
study of Empagliflozin and Linagliptin was found in concentration range of 10μg-50μg and 20μg-100μg and
correlation coefficient (r2) was found to be 0.999 and 0.999, % recovery was found to be 99.56% and 99.48%,
%RSD for repeatability was 1.2and 2.0, % RSD for intermediate precision was 1.1 and 1.1 respectively. Hence
the suggested RP-HPLC method can be used for routine analysis of Empagliflozin and Linagliptin in API and
Pharmaceutical dosage form.
Key Words: Empagliflozin, Linagliptin, RP-HPLC method, Linearity

The estimation of Emtricitabine and Tenofovir DF was done by RP-HPLC. The assay of Emtricitabine and Tenofovir DF was performed with tablets and the % assay was found to be 99.77 and 99.04 which shows that the method is useful for routine analysis. The linearity of Emtricitabine and Tenofovir DF was found to be linear with a correlation coefficient of 0.999 and 0.999, which shows that the method is capable of producing good sensitivity. The acceptance criteria of precision is RSD should be not more than 2.0% and the method show precision 0.22 and 0.5 for Emtricitabine and Tenofovir DF which shows that the method is precise.
Key words: Emtricitabine, Tenofovir, RP-HPLC

Aim of the study is pharmacological screening of leaves of Tamarindus indica for anticonvulsant activity.
Pentylenetetrazole-induced seizure and Maximal electroshock-induced seizure study were used to evaluate
anticonvulsant activity. Results obtained in this study indicate that the ethanol leaf extract of Tamarindus indica
possesses bioactive principles that have anticonvulsant activity and that Tamarindus indica is a partial neuromuscular
agonist.
KEY WORDS: Tamarindus indica, Pentylenetetrazole-induced seizure, Maximal electroshock-induced seizure.

A simple and selective LC method is described for the determination of Isoniazid and Rifampicin in tablet dosage
forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of
45 volumes of acetonitrile and 55 volumes of mixed phosphate buffer with detection of 225 nm. Linearity was
observed in the range 36-84 μg /ml for Isoniazid(r2 =0.9987) and 30-70μg /ml for Rifampicin (r2 =0.9977) for the
amount of drugs estimated by the proposed methods was in good agreement with the label claim.
The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three
different levels. Recovery experiments indicated the absence of interference from commonly encountered
pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing
%RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of
pharmaceutical dosage form.
KEY WORDS: Isoniazid, Rifampicin, tablet dosage forms, RP-HPLC method

RP-HPLC method was developed for the simultaneous estimation of Paracetomol, Domperidone and Flunarazine
in pharmaceutical bulk drugs and tablet dosage forms. A wavelength 210nm was selected and the mobile phase
consists of water and methanol in 50:50% v/v ratios at a flow rate of 1 ml/min were found to be optimum
conditions for analysis. the limits of % recovered Shown be in the range of 98-102% the results obtained for
Paracetomol, Domperidone Maliate And Flunarizine were found to be within the limits. Hence the method was
found to be accurate. The limits of % recovery of drugs were 98-102%. Calibration curve was plotted and
correlation co-efficient for the drugs Paracetomol, Domperidone Maliate and Flunarizine found to be 0.999, 0.999
and 1.000. Hence the developed method could be used for simultaneous estimation of Paracetomol, Domperidone
Maliate and Flunarizinein pharmaceutical dosage forms.
KEY WORDS: RP-HPLC method, Paracetomol, Domperidone Maliate and Flunarizine

A simple and selective LC method is described for the determination of Emtricitabine, Rilpivirine and Tenofovir
dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a
mixture of mixed Phosphate buffer pH: 4: Acetonitrile (40:60v/v/v), with detection of 262nm. Linearity was
observed in the range 32.5-97.5 μg /ml for Emtricitabine (r2 =0.9976) 40-120μg/ml for Rilpivirine (r2 =0.996)& 2-
6μg /ml for Tenofovir (r2 =0.993) for the amount of drugs estimated by the proposed methods was in good
agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed
by recovery studies at three different levels. Recovery experiments indicated the absence of interference from
commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the
repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be
used for routine analysis of pharmaceutical dosage form.
KEY WORDS: Liquid chromatography (LC), RSD Relative standard deviation,r2 correlation coefficient.

The aim of the present study was to develop and optimize immediate release tablets of model drug (Carvedilol)
to give quick onset of action with better patient compliance. In such cases, bioavailability of drug is significantly
greater and adverse event is reduced than those observed from conventional tablet dosage form. By performing
compatibility studies by IR spectrophotometry, no interaction was confirmed. Immediate release tablets were
formulated by direct compression method and evaluated by UV-Visibile spectrophotometer. Standard calibration
curve prepared to determine the drug content in the prepared tablets. Prior to compression, the blend of drug and
excipients were evaluated for flow properties such as Angle of repose, Bulk density, Tapped density, %
Compressibility, and Hausner ratio. All the formulation showed excellent properties. Immediate release tablets
were prepared by direct compression technique using CADMACH 16 station tablet punching machine, equipped
with round flat punches of 8 mm diameter. Post compression evaluation of prepared oral disintegrating tablets
were carried out with the help of different pharmacopoeial and non-pharmacopoeial (industry specified) tests.
The shape and color of all the formulations were found to be circular and white in color. The thickness was
found to be uniform in specific formulations. The hardness and friability are also within the permitted limits.
Dissolution of tablets was carried out. The croscaramellose sodium used formulation gave the more dissolution
profile compared to other superdisintegrants.
KEY WORDS: Carvedilol, immediate release tablets, croscaramellose sodium

The Sustained released tablets containing Valsartan SR tablets were successfully prepared by wet granulation
method. The optimized formulation contains the average thickness of 3.95±0.89, average hardness of 6.4±0.60,
average weight of 302.4±0.54, friability of 0.37.The optimized formulation F8 which releases the valsartan in
sustained manner in 1st hour it releases 8.51 % but the remaining drug release was sustained up to 12 hours.
KEY WORDS: Valsartan, wet granulation, sustained release tablets

A simple and selective LC method is described for the determination of Naltrexone and oxycodone in tablet
dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a
mixture of 30 volumes of ammonium acetate buffer,40 volumes of acetonitrile and 30 volumes of Methanol
with detection of 212 nm. The proposed methods were validated. The accuracy of the methods was assessed by
recovery studies at three different levels. Recovery experiments indicated the absence of interference from
commonly encountered pharmaceutical additives.
KEY WORDS: Naltrexone, oxycodone, bulk dosage form

A simple and selective LC method is described for the determination of torsemide and eplerenone in tablet dosage
forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of 55
volumes of Mixed Phosphate Buffer and 45 volumes of Acetonitrile with detection of 261 nm. Linearity was
observed in the range 5-15 μg/ml for torsemide (r2 =0.999) and 12.5-37.5μg /ml for eplerenone (r2 =0.998) for the
amount of drugs estimated by the proposed methods was in good agreement with the label claim.
KEY WORDS: Torsaemide, eplerenone, HPLC method