A simple and selective UPLC method is described for the determination of Topiramate Chromatographic
separation was achieved on a c18 column using mobile phase consisting of a mixture of 80 volumes of
Methanol and 20 volumes of Water with detection of 276 nm. Linearity was observed in the range 50-150 μg
/ml for Topiramate (r2 =0.998) for the amount of drugs estimated by the proposed methods was in good
agreement with the label claim. The proposed methods were validated. The accuracy of the methods was
assessed by recovery studies at three different levels. Recovery experiments indicated the absence of
interference from commonly encountered pharmaceutical additives. The method was found to be precise as
indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the
methods and can be used for routine analysis of pharmaceutical dosage form.

A simple and selective UPLC method is described for the determination of Phentermine. Chromatographic
separation was achieved on a c18 column using mobile phase consisting of a mixture of 40 volumes of
Methanol, 40 volumes of Acetonitrile and 20 volumes of Water with detection of 263 nm. Linearity was
observed in the range 50-150 μg /ml for Phentermine (r2 =0.990) for the amount of drugs estimated by the
proposed methods was in good agreement with the label claim. The proposed methods were validated. The
accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments
indicated the absence of interference from commonly encountered pharmaceutical additives. The method was
found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data
proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.

A simple and selective HPLC method is described for the determination of Mirabegron in tablet dosage forms.
Chromatographic separation was achieved on a Waters Acquity C18 (50mm x2.1 mm ID) 1.8μm using mobile
phase consisting of a mixture of 70 volumes of Potassium di-hydrogen phosphate and 30 volumes of methanol
with detection of 254nm. Linearity was observed in the range 50-120 μg /ml for Mirabegron (r2 =0.998) for the
amount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposed
methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels.
Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical
additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less
than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical
dosage form.

A new precise, accurate, rapid method has been developed for the estimation of Valbenazine pharmaceutical
dosage form by HPLC. From results the proposed method is highly sensitive, precise and accurate and it
successfully applied for the quantification of API content in the commercial formulations of Valbenazine
Educational institutions and Quality control laboratories A simple and selective HPLC method is described for
the determination of Valbenazine Chromatographic separation was achieved on a Phenomenex C18 (250×4.6
×5μ) using mobile phase consisting Acetonitrile : Water : Triethylamine buffer (60: 40: 0.5%) v/v with
detection of 264 nm. Linearity was observed in the range 50-150 μg /ml for Valbenazine (r2 =0.999) for the
amount of drugs estimated by the proposed methods was in good agreement with the label claim.

A simple and selective LC method is described for the determination of niacin and lovastatin in tablet dosage
forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture
of 55 volumes of water and 45 volumes of Methanol with detection of 240nm. Linearity was observed in the
range 20-100 μg/ml for niacin (r2 =0.998) and 10-30 μg /ml for lovastatin (r2 =0.993) for the amount of drugs
estimated by the proposed methods was in good agreement with the label claim. The proposed methods were
validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery
experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The
method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All
statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage
form.

The main objective of the study was to formulate and evaluate oral fast dissolving film containing Quetiapine
fumarate . Compatibility of Quetiapine fumarate with polymers was confirmed by FT-IR studies. Seven films
were evaluated for weight variation and thickness showed satisfactory results. Folding endurance of the films
was increased with increase in the concentration of polymer due to increase in the elasticity nature of the
polymer. Disintegration time of the films was increased with increase in the concentration of the polymer, as
more fluid is required to wet the film in the mouth. The presence of disintegrant showed a considerable effect
on the disintegration time of the films. Content uniformity study showed that the drug is uniformly distributed
in the film. The accelerated stability studies of the optimized FV formulation indicates that the formulated
oral fast dissolving films were unaffected after 3 months storage under accelerated conditions as there were
no signs of visually distinguishable changes in appearance, disintegration time and cumulative percentage of
drug release.From the present investigation it can be concluded that oral fast dissolving film formulation can
be a potential novel drug dosage form for pediatric, geriatric and also for general population.

The method described by Touitou was employed with little modification for the preparation of various
ethosomal formulations containing different concentration of ethanol (20 % to 50 %) with sonication . The
techniques used were simple and reproducible. The prepared ethosomes were spherical and discrete in shape.
However ethosomes prepared by sonication method were more uniform and small in size which is essential
for skin penetration. While comparing the entrapment efficiency, ethosomes containing 30% w/w IPA and
prepared by soncation showed highest value respect to all other formulation; so it is concluded ethosomal
prepared by sonication and containing 30 % w/w IPA as the best formulation considering all other aspects
Increase in the polymer concentration led to increase in, % Drug entrapment efficiency, Particle size. The
invitro drug release decreased with increase in the polymer and copolymer concentration.Among all
formulations F6 shows Maximum drug release in 1440 min when compared with other formulations. Analysis of
drug release mechanism showed that the drug release from the formulations followed the Non fickian
diffusion mechanism and follows First order kinectics.Based on the results of evaluation tests formulation
coded F6 was concluded as best formulation.
Key Words: Ethosomes , Sonication , Transdermal ,Entrapment , Stability

Emulsion cross linking method can be successfully employed to fabricate Sitagliptin microspheres by heat
stabilization method. The technique provides characteristic advantage over conventional microsphere method,
which involves an “all-aqueous” system, avoids residual solvents in microspheres. Other methods utilize
larger volume of polymer, uneasy in dropping through syringe , air pollution, toxicity and difficult to remove
traces during filtration .FT-IR spectra of the physical mixture revealed that the drug is compatible with the
polymers and copolymer used. Micromeritic studies revealed that the mean particle size of the prepared
microspheres was in the size range 664-903μm for ionotropic gelation method and 511- 826 μm for
emulsion cross linking method,size of ionotropic gelation have high mean partice size than emulsion cross
link method and are suitable for bioadhesive microspheres for oral administration.Increase in the polymer
concentration led to increase in % Yield, % Drug entrapment efficiency, Particle size, % swelling and %
Mucoadhesion.The in-vitro mucoadhesive study demonstrated that microspheres of Sitagliptin using chitosan
as polymer and glutaraldehyde as cross linking agent adhered to the mucus to a greater extent than sodium
alginate along with Carbopol934.The invitro drug release decreased with increase in the polymer and
copolymer concentration.The invitro drug release shows almost same result for T3 and T7 but T7 of emulsion
cross linking method was optimized based on optimum swelling index, percentage mucoadhesion , drug
entrapment and drug relaese.
Key Words: Sitagliptin, microspheres, Ionotropic gelation method, Carbopol

Pharmacy is one of the healthcare professions that have significant role to play in health care system.
Pharmacists are academically qualified to become specialist in proper use of medications and therapy. As such,
their function can evolve to include evaluating, educating and advising patients to maintain the appropriate use
of medicine and to provide patients with pharmaceutical care treatments. According to recent systemic reviews,
community pharmacist giving counseling services improved clinical outcomes. In some countries, it is
mandated by law and regulation that to give the major priority for community pharmacist in modern health care
system. Pharmaceutical care is the responsible provision of drug therapy for the purpose of achieving definite
outcome which improve patient quality of life. It seems that there is a lack of accurate and scientific knowledge
is one of the basic weakness of most community pharmacists leading to inappropriate practice, this can be
overcome by training programs such as educational pamphlets and continuing educational seminars play
important roles in increasing pharmacists’ knowledge and therefore improves their performance in practice.
Keywords:
Pharmaceutical Care, Continuing Educational Programs, Pharmacy Practice, Community Pharmacist, Hospital Pharmacist.

Pharmacy is one of the healthcare professions that have significant role to play in health care system. India is
endowed with a long coastline and hence offers scope for large exploitation of marine wealth. The occurrence
of antimicrobials in fish and fisheries tissues has received broad interest over the last years. Reports recently
published have demonstrated that continuous exposures to these compounds may result in accumulation of the
parent compound, their metabolites or both in tissues of aquatic organisms. Antimicrobials (Tetracyclines,
Sulphonamides, Chloramphenicol, Nitrofurans, Fluoroquinolones, Endosulphan and Nitroimidazoles) are
widely prescribed for therapeutic and prophylactic reasons against microbial infections and also in animal farms
as growth promoting agents. The presence of these drugs in animal tissues can have undesirable effects on
consumer health, such as allergies, but that is not the main problem because there is a low incidence of such
cases; the main problem is that uncontrolled ingestion of antimicrobials by consumer causes the development of
bacterial resistance, which translates into a much bigger problem for consumers health when dealing with
infections
Key words: Tetracyclines, Sulphonamides, Chloramphenicol, Nitrofurans, antibiotic resistance, sea foods.