ABSTRACT
The species of Polycarpaea are used for many medicinal purposes such as in diabetes, reduction in cholesterol
level, anti microbial action, hepatoprotective activity etc. Preliminary phytochemical screening for successive
solvent extracts, revealed the presence of alkaloids, tannins, carbohydrates, sterols, gums and mucilage. Anti
inflammatory studies using HRBC (membrane stabilization) and protein denaturation methods.
Key words: Polycarpaea aurea, anti inflammatory, protein denaturation, HRBC.

ABSTRACT
Thiadiozoles and Azetidinones are important heterocyclic molecules employed in field of research and
development of newer therapeutic agents. The work focused to the antimicrobial action of azetidiones moiety
(β-lactum), due to its greater resistance to enzymatic cleavage by lactomases. Various Azetidinone derivatives
were synthesized via thiadiazole intermediate and screened for antimicrobial activity by agar well diffusion
method using gram positive (Bacillus subtilis) and gram negative (Escherichia coli) organisms at 400μg/well
and 200μg/well. The compound TDPCB (Thiadiazole-para-chloro-benzaldehyde-substituted azetidinone
derivative) was found to have significant antimicrobial property.
Key words: Azetidinone, Thiadiazole, substituted aldehydes, antibacterial.

The present study was aimed to investigate the comparative study on hepatoprotective activity of alcoholic
extracts from Mollugo pentaphylla and Mollugo cerviana against carbon tetrachloride induced hepatotoxicity in
rats. Most of the hepatotoxic chemicals damage liver cells mainly by inducing lipid peroxidation and other
oxidative damages. Liver function was assessed by the determination of serum glutamate oxaloacetate
transaminase(SGOT), serum glutamate pyruvate transaminase( SGPT) ,Alkaline phosphatase (ALP), Acyl
carrier protein (ACP), bilirubin. The investigation reveals that the alcoholic extract of the M. pentaphylla and
M. cerviana exhibiting a significant protective from liver damage in Carbon tetrachloride (CCl4)induced liver
damage model. The present study involve of comparison of pharmacological activity of two plant species.
Silymarin (25mg/kg) is used as positive control. The presence of flavonoid compound in both of the extract
may be responsible for the significant hepatoprotective property.
Key words: Hepatoprotective Property, Mollugo pentaphylla, Mollugo cerviana.

Hibiscus micranthus, (Malvaceae) is a shrubby, erect, branched, slender and stellately hairy plant, widely
distributed in hotter parts of India, Ceylon, Saudi Arabia and tropical Africa. The plant is reported to contain
phytoconstituents such as phenolic acids, flavonoids, β-sitosterol, fatty alcohols, alkanes and acids. Its leaves ,
stem, flowers, root has been used in the Indian traditional system as a medicine to treat various diseases..
Traditionally the plant is considered as a valuable febrifuge in India and is used as antipyretic, antiinflammatory,
antifungal, antiviral, antitumor, hypoglycemic. Various research studies proved that the different
parts of hibiscus micranthus possess diverse biological activities such as antipyretic, anti-inflammatory,
antitumor ,haematological effects, antimicrobial, antiviral, antifertility.The present paper is an overview on its
pharmacognostical, phytochemical and pharmacological properties reported in the literature.
Key words: Hibiscus micranthus, pharmacognostical, phytochemical, pharmacological properties.

A simple, Accurate, precise method was developed for the simultaneous estimation of the Cobicistat and
Atazanavir in Tablet dosage form. Chromatogram was run through ODS (250mm 4.6mm, 5μ). Mobile phase
containing Buffer and Acetonitrie in the ratio of 45:55A was pumped through column at a flow rate of 1ml/min.
Temperature was maintained at 30°C. Optimized wavelength for Cobicistat and Atazanavir was 270nm.
Retention time of Cobicistat and Atazanavir were found to be 5.023 min and 3.579 min. %RSD of the
Cobicistat and Atazanavir were and found to be 1.6 and 0.31 respectively. %Recover was Obtained as 100.3%
and 100.21% for Cobicistat and Atazanavir. LOD, LOQ values were obtained from regression equations of
Cobicistat and Atazanavir were 0.87 ppm, 1.64 ppm and 1.33ppm, 4.17 ppm respectively. Regression equation
of Cobicistat is y = 4985.x + 1820, and of Atazanavir is y = 33657x + 698.2.
Key words: Cobicistat, Atazanavir, RP-HPLC.

A new method was established for estimation of Uridine Triacetate by RP- HPLC method. The retention times
were found to be 2.425mins. The % purity of Uridine Triacetate was found to be 99.94%.The system suitability
parameters for Uridine Triacetate such as theoretical plates and tailing factor were found to be 4187, 1.5. The
analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study of Uridine
Triacetate was found in concentration range of 20μg-100μg and correlation coefficient (r2) was found to be
0.999, % recovery was found to be 99.95%, %RSD for repeatability was 0.2, % RSD for intermediate precision
was 0.1. The precision study was precision, robustness and repeatabilty.LOD value was3.06 and LOQ value was
10.14.
Key words: Uridine Triacetate, RP- HPLC method, Pharmaceutical dosage form.

Aim of the Study is to develop simple, rapid, specific and sensitive RP-HPLC method for the determination of
Ornidazole and Ofloxacin. The Specificity of Ofloxacin and Ornidazole was found there was no interference in
the method and good separation between all peaks; it means no impurity was interfered and also reveals that
commonly used excipients and additives present in the pharmaceutical formulation were not interfering in the
proposed methods. The precision was found to be within the limits. The limit were not more than RSD
<2%.Precision RSD was found to be 0.9937for Ofloxacin and 0.8048 for Ornidazole.The low %RSD value for
intraday and inter day precision reveled that the proposed method is robust and rugged. From the linearity table
it was found that , the drug obeys beer’s law and from the linearity range 22 to 66ug/ml for ofloxacin and 55 to
165ug/ml for ornidazole. In Robustness parameter in both conditions the RSD was less than 2%. Hence the
method was better for pharmaceutical formulation analysis.
Key words: Ornidazole and Ofloxacin, sensitive RP-HPLC method.

A simple, selective, rapid, precise and economical reverse phase high performance liquid chromatographic
(RP-HPLC) method has been developed for the simultaneous estimation of Paracetamol (PARA) and
Flupiritine Maleate (FLU) from pharmaceutical formulation. The method is carried out on Agilent C18 (25
cm x 4.6 mm i.d., 5 μ) column with a mobile phase consisting of Methanol Water (0.2% TEA, adjusted to pH
3 using orthophosphoric acid) in the ratio of 50:50 v/v. The retention time of Paracetamol and Flupiritine
Maleate is 6.3 min and 4.2 min respectively with the flow rate of 1mL/ min with PDA detection at 240 nm.
The linear regression analysis data for the linearity plot showed good linear relationship with correlation
coefficient value for Paracetamol and Flupiritine Maleate were R2=0.9998 and R2=1.0000 in the
concentration range of 1.25-60.08 μg. mL-1 , 6.25-300.03 μg. mL-1 respectively. The relative standard
deviation for intra-day precision has been found to be lower than 2.0 %. The method is validated according to
the ICH guidelines. The developed method is validated in terms of specificity, selectivity, accuracy,
precision, linearity, limit of detection, limit of quantitation and solution stability. The proposed method can
be used for simultaneous estimation of these drugs in marketed dosage forms.
KEY WORDS: RP-HPLC, Paracetamol and Flupiritine Maleate

A new method was established for simultaneous estimation of Bicalutamide and Atenolol. The
chromatographicconditionsweresuccessfullydevelopedfortheseparationofBicalutamide and Atenolol by using
Agilent C18 5μm (4.6*250mm) column, flow rate was 1ml/min, mobile phase ratio was Water: ACN
(70:30%v/v), detection wavelengthwas 254nm.
KEY WORDS: AgilentC18, Bicalutamide and Atenolol, RP-HPLC method.

Dexlansaprazole is a proton pump inhibitor, belongs to group of benzimidazole, used for the treatment of
gastric and duodenum ulcers. Dexlansaprazole undergoes degradation in acid medium of the stomach, can be
coated with enteric coating polymer that will safely deliver the drug in the small intestine. In this present study
an attempt was made to formulate and evaluate Dexlansaprazole as enteric coated tablet. Delayed release
tablets of Dexlansaprazole were prepared by wet granulation method using HPMC K4M and HPMC K15M,
Avicel PH 102 (MCC) as filler and starch as binder. The prepared tablets were evaluated for hardness,
weightvariation, friability and drug content uniformity and it was found that the results comply with official
standards. The prepared tablets were coated using enteric coating polymer such as cellulose acetate phthalate,
EudragitL100 by dip coating method. The invitro release was studied using pH 1.2 acidic buffer and pH6.8
phosphate buffer. The in vitro release study revealed that the prepared tablets were able to sustain release drug
into the intestine. The release kinetics studies Showed that the release was zero order diffusion controlled and
the nvalues obtained from the Korsmeyer-Peppas model showed that the release mechanism was supercase-II
transport. Stability studies indicated that the developed tablets were stable and retained their pharmaceutical
properties at room temperature and 40°C/75 %RH for a period of 1month.
Key words: Dexlansaprazole, Delayed release, HPMC.

Metformin HCl sustained release matrix tablets were prepared by direct compression. All the tablets were
prepared under identical conditions to minimize the processing variables. Tablet formulations were further
evaluated for physical parameters. It was revealed that all the tablet formulations were found to be stable and
meeting I.P specified limits for weight uniformity, friability, drug content. Drug content estimated for all the
tablet formulations were highly uniform with less than 2.5% variation. Drug content was also the same in case of
matrix tablets containing polymers. The matrix tablet formulations prepared with drug and polymer of guar gum
in F5 could be suitable for extending the drug release more than 12 hrs.
Key words: Metformin HCl, sustained release matrix.