The chronological behaviour of Hypertension confirms increased blood pressure at early morning hours, need a preferable dosage form which will provide desired concentration of the drug at pre-determined time points especially in early morning hours. Pulsatile dosage forms are designed to mimic the circadian rhythm by releasing the drug at the desired time, by means of an internal preprogrammed designed dosage form that is initiated when the dosage form comes in contact with gastrointestinal fluids especially in colon. The prepared dosage forms were optimized and evaluated both in vitro and in vivo evaluation. Metoprolol tartarate, a selective beta blocker pulsincaps were formulated using, body of the capsules with modified solubility by coating with formaldehyde and polymer plug of Eudragit L 100 was fitted in mouth of body of the capsule. The results of the final two optimized formulation P1 and P2 was found to ideal for pulsatile release. The maximum in vitro drug release of 101.24± 1.24 and 101.08± 1.54 % (first pulse Metoprolol tartarate granules immediately release the drug after dinner from the uncoated cap of the capsule), 94.84±1.4% and 97.74±1.8 (Second pulse at early morning hours after lag time in Colonic pH). The selected formulations of Metoprolol tartarate pulsincaps (P1 and P2) were subjected for pharmacokinetic studies using male albino rats. From the Pharmacokinetic studies, in-vivo studies shown that increased AUC, relative bioavailability and delayed time of Metoprolol tartarate pulsincap proves the Sustainability and targetability of drug in colon.

The aim study is to evaluate phytoconstituents and in vitro anticancer activity of immature bark of Aegle marmelos. Total phenol content of extract was found to be 898mg/g. Chloroform extract showed potent anticancer activity in VERO, 3T3-L1 cell lines.

An α-amylase inhibitor acts as an anti-nutrient that obstructs the digestion of starch and absorbtion of glucose. Acarbose is a complex oligosaccharides that delays the digestion of carbohydrates, thereby resulting in a smaller raise in blood glucose concentration following food intake. Acarbose, inhibit the action of pancreatic amylase in breaking down starch, thereby achieving this effect. Our finding reveals that Aegle marmelos extracts (CAM & PEAM) efficiently inhibits -amylase suggesting that Aegle marmelos extract Is a starch blocker. α-glucosidase inhibitors retard the digestion of carbohydrates to simpler carbohydrates and slows down the absorbtion of the later in the small intestine. Thereby, preventing high glucose concentration in the blood after a meal. The antidiabetic action of Aegle marmelos extracts is attributed to the intestinal α-glucosidase inhibitory activity. In conclusion it may be stated that, there occurs selective decrease in the hyper glycemic state after the administration of the Aegle marmelos extract.

The objective of this research work is to develop a pH independent sustained release drug delivery system for a model drug propranolol hydrochloride basic drug to overcome the decreased bioavalibility and to avoid variation of drug release at various gastro intestinal pH. Organic acids such as succinic acid, maleic acid and fumaric acid were used as release modifiers. In case of formulations without any release modifier, the extent and rate of drug release at pH 1.2 was much higher than that of at pH 7.4. Different formulations were prepared by using release modifiers in different amount in order to overcome the pH dependent solubility. The formulation were subjected to various evaluation parameters such as hardness, friability, assay and in-vitro release studies. An in vitro release study was carried out in two different pH Medias such as pH 1.2 and pH 7.4. Through in-vitro release studies it was found that formulation containing 80 mg of fumaric acid provided better drug release compared to formulation containing maleic acid and succinic acid. The selected formulations were subjected to stability studies. Finally, it was concluded that propranolol hydrochloride could be formulated as a pH- independent matrix tablet using fumaric acid as a release modifier.

RP-HPLC method was developed and validated as per ICH guidelines for the estimation of Ambroxol.HCl and Levocetirizine dihydrochloride. Simultaneous estimation of Ambroxol.HCl and Levocetirizine dihydrochloride were carried out by RP- HPLC using sodium phosphate buffer (pH = 3.0): Methanol (30:70) and column Phenomenex Luna C18 (250 x 4.6 mm, 5μm) as a stationary phase and peak was observed at 230 nm which was selected as a wavelength for quantitative estimation. It was validated for specificity, linearity, precision, accuracy, robustness and ruggedness studies. Results reveals that recovery value of pure drug were between 98 % to 102 % which indicates that the method is accurate and also reveals that commonly used excipients and additives present in the pharmaceutical formulations were not interfering in the proposed methods. Based on the results observed, it was concluded that proposed method can be used for routine analysis of Ambroxol HCl and Levocetirizine dihydrochloride.

A simple, sensitive, and precise high performance liquid chromatographic method for the determination of related substances of Lacosamide in pharmaceutical formulation has been developed and validated. The Impurities were well separated on a Inertsustain HP C18 (100mm X 4.6mm, 3μm) by the gradient program using Phosphate buffer (pH 2.0) and Acetonitrile at a flow rate of 1.0 mL /min with detection wavelength at 210 nm. The method is considered ‘accurate’ if the individual % recovery of Lacosamide and its impurities at each level should not be less than 90.0% and should not be more than 110.0%.

The aim of the present work was to develop and validate a simple, efficient, economical method for the estimation of drug Ziprasidone hydrochloride in its pellets by reverse phase high pressure liquid chromatography. Chromatography was performed on Inertsil ODS C18 column (150 mm x 4.6 mm, 5 μm) with mobile phase containing methanol: 0.05%v/v ortho-phosphoric acid in water (90:10) at a flow rate of 1 mL/min and eluents were monitored at 270 nm. The retention time of drug was 3min and showed a good linearity in the concentration range of 20-60 μg/mL with a correlation coefficient of 0.9998.The developed method was validated for specificity, accuracy, precision, recovery, linearity, robustness, and system suitability and stability. The low standard deviation values and good recoveries indicate the reproducibility and accuracy of the developed method. By the overall results obtained, it was concluded that the developed method was more accurate, precise, specific and robust with ±5º C in temperature, ± 10% in flow rate.The percentage of recovery of ziprasidone was found to be 99.8% at 100.1% level.The method could be successfully used for the analysis of ziprasidone in bulk and dosage forms.

The present study indicating the presence of antiurolithiatic effect in Hibiscus vitifolius Linn. Aerial parts against EG induced urolithiasis mediated possibly through a combination of CaOx crystal inhibition, diuretic, antioxidant effects. Its medicinal use for urinary pathologies in the Indian folk medicine has been included as an antiurolithiatic drug. The results indicate that the administration of ethanolic extract of Hibiscus vitifolius Linn. significantly reduced the growth of urinary stones.the underlying mechanism could be due to its diuretic effect, antioxidant effect, nephroprotective property and lowering the concentration of urinary stone forming constituents and it may prove to be effective for the treatment of kidney stones.

The aim of the present work was to develop and validate a simple, efficient, economical method for the estimation of Rosuvastatin and its related substances in bulk and dosage forms by reverse phase high pressure liquid chromatography. Chromatography was performed on X-Terra -C18 (150 mm x 4.6 mm, 3.5μm) with mobile phase containing Mobile phase - A: pH 3.00 KH2PO4 buffer: Methanol (80:20 v/v) and Mobile phase - B: pH 3.00 KH2PO4 buffer: Methanol: Acetonitrile (25:15:60 v/v)at a flow rate of 1mL/min and eluents were monitored at 248nm. The retention times of Rosuvastatin,Anti-Isomer, 5-Oxo and Lactone impurities were 23.538min, 25.634,28.546 and 29.344min respectively and showed a good linearity in the concentration range of 0.3-7.5 μg/mL for Rosuvastatin, 0.2-7.5μg/mL for Anti-Isomer, 0.2-7.5μg/mL for 5-Oxo and 0.2-7.5μg/mL for Lactone impurities with a correlation coefficient of 0.9998, 0.9996, 0.9992 and 0.9999 respectively. The validation characteristics included specificity, linearity, and limit of detection, limit of quantification, precision, robustness and stability. Validation acceptance criteria were met in all cases. The percent recoveries ranged between 85-115%, RSD < 2%. The method could be successfully used for the analysis of Rosuvastatin and its related substances in bulk and dosage forms.

A simple, sensitive, and precise high performance liquid chromatographic method for the determination of related substances of Lacosamide in pharmaceutical formulation has been developed, validated and used for the determination of related substances in commercial pharmaceutical products. The developed method was found to be specific, precise, linear, accurate, rugged and robust. LOQ Values for all the known impurities were below reporting thresholds. The method was validated for specificity, linearity, accuracy, precision, robustness and solution stability. Recovery was found to be in the range of 90-110%. The validation parameters and recovery studies were carried out and reported. The obtained results were satisfactory and good agreement as per the ICH guidelines

Tremendous progress has been made in basic cancer biology and in the development of novel cancer treatments; cancer remains a leading cause of death in the world. Over the last few years, researchers have aimed at identifying and validating plant derived substances for the treatment of various diseases. There have been vast discoveries of potent cytotoxic agents attributed to Asian and Ayurvedic Indian traditional medicine. By increasing research into herbal drugs, there is a hope to identify the most promising agents and understand their many actions. The present review aims to explore medicinal values of Aegle Marmelos, against cancer and various diseases and also suggest some methods for screening anticancer agents.