ABSTRACT
Medicinal plants often form an important constituent of many formulations used by the folk medical
practitioners. Tiliacora acuminata of Menispermaceae family is such a medicinal plant largely available in
India. It is considered as an ingredient in many of the ayurvedic preparations. It is also regarded as an antidote
for snake bite. As most parts of the plant have various pharmacological actions, this plant is known to be a
wonder plant. The objective of this review is to understand the extend of phytochemical and pharmacological
evaluations carried out on this plant.
Key words: Menispermaceae, Krsnavetra, Antibacterial, Tiliasine

Onychomycosis is a fungal infection of the nail bed or nail plate that accounts for approximately 50% of all
nail diseases and is the most common disorder in adults. Onychomycosis though rarely life threatening, they
can be very painful, discomfortable and disfiguring for the sufferer and may produce serious physical and
occupational limitations, psychological and emotional effects, and affect quality of life. Deformed nails can
lead to surrounding tissue damage and may promote secondary bacterial infection. The objective of the present
work was to formulate a medicated antifungal nail lacquer containing itraconazole for the treatment of
Onychomycosis. In the present study, nail lacquer containing a permeation enhancer salicylic acid and release
extender ethyl cellulose in different concentration are tried out and comparison of extent of drug permeation
has been done among the same. Then, the formulated nail lacquers were compared for physical appearance,
drying time, nonvolatile content, gloss, water resistance, drug content, drug diffusion and anti -microbial
studies.
Key words: Onychomycosis, Itraconazole, nail lacquer

A simple and selective LC method is described for the determination of Lumefantrine and Artemether in tablet
dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a
mixture of Phosphate buffer (KH2PO4) Ph3.5: Methanol (30:70v/v),with detection of 238 nm. Linearity was
observed in the range 16-24 μg /ml for Artemether (r2 =0.995) and 72-168μg /ml for Lumefantrine (r2 =0.997)
for the amount of drugs estimated by the proposed methods was in good agreement with the label claim The
proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three
different levels. Recovery experiments indicated the absence of interference from commonly encountered
pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis,
showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine
analysis of pharmaceutical dosage form.
Key Words: Lumefantrine, Artemether, RP HPLC method

A reverse phase-liquid chromatographic method with UV detection at 225nm is described for simultaneous
determination of Alprazolam and Sertaline. Chromatographic separation of the three drugs was achieved on an
inertsil column, C18 (150x4.6 ID) 5μm column using a mobile phase consisting of a mixture of Ammonium
Acetate buffer (pH 3.5): ACN: ACN in a ratio of 30:70. The liquid chromatographic method developed offers
symmetric peak shape, good resolution, and reasonable retention time for both drugs. Linearity was found to
be acceptable over the concentration ranges 1-3μg/ml for Alprazolam and 100-300μg/ml Sertaline. Accuracy
was found to be acceptable over the concentration ranges 1-3μg/ml for Alprazolam and 100-300μg/ml
Sertaline. The liquid chromatographic method was successfully applied to the quality control of formulated
products containing Alprazolam and Sertaline.
Key Words: Alprazolam, Sertaline, reverse phase-liquid chromatographic method

A simple and selective LC method is described for the determination of Apixaban dosage forms.
Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of
HPLC water (pH 5.8): ACN (55:45v/v), with detection of 281 nm. Linearity was observed in the range 75-150
μg /ml for Apixaban (r2 =0.994) for the amount of drugs estimated by the proposed methods was in good
agreement with the label claim. The proposed methods were validated. The accuracy of the methods was
assessed by recovery studies at three different levels. Recovery experiments indicated the absence of
interference from commonly encountered pharmaceutical additives. The method was found to be precise as
indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the
methods and can be used for routine analysis of pharmaceutical dosage form.
Key Words: Apixaban, LC method, pharmaceutical dosage form

A simple and selective LC method is described for the determination of Ibuprofen and Tramadol in tablet
dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a
mixture of 30 volumes of ammonium acetate buffer, 40 volumes of acetonitrile and 30 volumes of Methanol
with detection of 238 nm. Linearity was observed in the range 60-140 μg/ml for Ibuprofen (r2 =0.999) and 6-
14 μg /ml for Tramadol (r2 =0.996) for the amount of drugs estimated by the proposed methods was in good
agreement with the label claim. The proposed methods were validated. The accuracy of the methods was
assessed by recovery studies at three different levels. Recovery experiments indicated the absence of
interference from commonly encountered pharmaceutical additives. The method was found to be precise as
indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the
methods and can be used for routine analysis of pharmaceutical dosage form.
Key Words: Liquid chromatography (LC), RSD Relative standard deviation, r2 correlation coefficient

A simple and selective LC method is described for the determination of Deferiprone dosage forms.
Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of
Triethylamine (pH 3.5): ACN (50:40v/v), with detection of 280 nm. Linearity was observed in the range 125-
375 μg /ml for Deferiprone (r2 =0.994) for the amount of drugs estimated by the proposed methods was in
good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was
assessed by recovery studies at three different levels. Recovery experiments indicated the absence of
interference from commonly encountered pharmaceutical additives. The method was found to be precise as
indicated by the repeatability analysis, showing % RSD less than 2. All statistical data proves validity of the
methods and can be used for routine analysis of pharmaceutical dosage form.
Key Words: Deferiprone, % RSD, pharmaceutical dosage form

Aim is to develop a new RP-HPLC method for the estimation of Diacerein in pharmaceutical dosage form.
Linearity was observed in the range 20-120 μg /ml for Diacerein (r2 =0.996) for the amount of drugs estimated
by the proposed methods was in good agreement with the label claim. The above results reveal that method is
linear. The sample recoveries in all formulations were in good agreement with their respective label claims and
they suggested non-interference of formulation excipients in the estimation.
Key Words: Diacerein, pharmaceutical dosage form, RP-HPLC method

To investigate the anti Hyperlipidemic activity of methanol extract of Desmostachya bipinnata on
Triton x 100 and High Fat Diet induced Hyperlipidemia in male Wistar rats. In this model of
Hyperlipidemia, 30 adult male wistar rats (150-200gms) were evenly divided into 5 groups in both
groups. Group-1 and Group-2 served as untreated and model controls respectively, while Group-3, 4 and 5
were the treatments groups which were simultaneously treated with standard, 200 and 400 mg/kg
extract respectively along with High Fat Diet and Triton x 100. On last day, blood samples for biochemical
parameters, were obtained under inhaled diether anaesthesia. HFD and Triton x 100 treatment caused
Hyperlipidemia as evidenced by marked elevation in Cholesterol, Triglycerides, LDL, VLDL and
decrease in HDL levels. Co-administration of extract with HFD and Triton x 100 decreased rise
Cholesterol, Triglycerides, LDL, VLDL and increase in HDL levels. It was observed that the methanol
extract of Desmostachya bipinnata conferred Anti- Hyperlipidemia activity by biochemical observation
against HFD and Triton-x-100 induced Hyperlipidemia in rats. In the near future could constitute a lead to
discovery of a novel drug for treatment of drug induced Hyperlipidemia
Key words: Desmostachya bipinnata, Anti- Hyperlipidemia activity, methanol extract

An attempt was made to know the activity of Thespesia populnea leaves against the dandruff causing
organism Malassezia furfur. Thespesia populnea leaves were collected and their powered extracts were used
for the activity studies. The antidandruff activity of Thespesia populnea leaf extract using agar well diffusion
method was carried out. Sensitivity of the organism to herbal shampoo containing leaf extract was also
studied. Herbal shampoo formulation was prepared using Sodium lauryl sulphate as foaming agent of
concentrations 1 to 20%. Test parameters for shampoo like appearance, pH, and foaming ability, solid content,
viscosity, surface tension and stability test was evaluated. The formulation, F3 with 5% Sodium lauryl
sulphate was selected, based on its evaluation parameters. The results of this study showed that Thespesia
populnea extracts and its shampoo had antidandruff activity. The concentration chosen was 30% extract
because in that concentration the value of the resistor area is still categorized well. The stability test results
stated that all the formulas were stable even after 30 days of stability studies.
Key words: Thespesia populnea, Malassezia furfur, herbal shampoo, Anti-dandruff activity