The UV Spectrophotometric estimation was done by using Shimadzu 1700- UV Visible spectrophotometer.
The estimation of Pomalidomide is done by using Water as a solubilising agent and distilled water as the
solvent and the λ max was found to be 223nm for calibration curve method and first order derivative. The
proposed UV-Spectrophotometric methods were suitable method for the determination of Pomalidomide
dosage form. All the parameters of developed methods met the criteria of ICH guidelines for method
validation. The developed UV methods for the estimation of Pomalidomide are said to be rapid, simple,
precise, accurate, sensitive and cost effective and reproducible within the specified method parameter and can
be effectively applied for the routine analysis of Pomalidomide in bulk and formulations.
Key Words: Pomalidomide, UV-Spectrophotometric
Key Words: Deferiprone, pharmaceutical dosage form, LC method

A simple and selective LC method is described for the determination of Canagliflozin dosage forms.
Chromatographic separation was achieved on a c18 column using mobile phase consisting of a mixture of
Methanol: ACN: H2O (30:50:20v/v/v), with detection of 250 nm. Linearity was observed in the range 20-60
μg /ml for Canagliflozin (r2 =0.999) for the amount of drugs estimated by the proposed methods was in good
agreement with the label claim. The proposed methods were validated. The accuracy of the methods was
assessed by recovery studies at three different levels. Recovery experiments indicated the absence of
interference from commonly encountered pharmaceutical additives. The method was found to be precise as
indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the
methods and can be used for routine analysis of pharmaceutical dosage form.
Key Words: Canagliflozin, dosage form, LC method

Aim of the study is to evaluate the Hepatoprotective activity of ethanolic leaves extracts of Hibiscus
platanifolius. In present investigations leaf of Hibiscus platanifolius was subjected to proximate analysis and
showed significant values. In that the ethanolic extract (6.7w/w) shows more extractive value compared to
water extractive value (5.8w/w). The assessment of hepatoprotective activity was carried out by estimation of
various biochemical parameters i.e. Glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase
(GPT), alkaline phosphatase (ALKP), total bilirubin (TBL), total cholesterol (CHL), lactate dehydrogenase
(LDH), total protein (TPTN), and albumin (ALB) in serum. The biochemical observations were supported by
histological examination of liver sections of rats. Results showed significant Hepatoprotective activity of
ethanolic leaves extracts on tested dose 200mg/kg b wt and 400mg/kg b wt.
Key Words: Hibiscus platanifolius, Hepatoprotective activity, ethanolic leaves extracts

A simple, Precised, Accurate method was developed for the estimation of Clofarabine by RP-HPLC
technique. Chromatographic conditions used are stationary phase Symmetry C18 column 150 mm x 4.6 mm,
3.5., Use Buffer 0.1% ortho phosphoric acid : acetonitrile (30:70v/v) and flow rate was maintained at 1.0
ml/min, detection wave length was 219nm, column temperature was set as ambient and diluent was mobile
phase conditions were finalized as optimized method. System suitability parameters were studied by injecting
the standard five times and results were well under the acceptance criteria. Linearity study was carried out
between 10% to150 % levels, R2 value was found to be as 0.999. Precision was found to be 0.6 for
repeatability and 1.2 for intermediate precision. LOD and LOQ are 0.10μg/ml and1.00μg/ml respectively. By
using above method assay of marketed formulation was carried out 100.01% was present. Degradation
studies of Clofarabine were done, in all conditions purity threshold was more than purity angle and within the
acceptable range.
Key Words: HPLC Clofarabine. Method development. ICH Guidelines

The main objective of the study was to formulate and evaluate oral thin film containing drug Montelukast
Sodium. The 4 and 5 % W/V HPMC, PVA, CMC films were prepared by Solvent Casting Method.
Compatibility of Montelukast Sodium with polymers was confirmed by FT-IR studies. The presence of
disintegrant showed a considerable effect on the disintegration time of the films. Montaleukast sodium can be
prepared by solvent casting method. A 4% w/v of HPMC (fv) film exhibited required tensile strength, folding
endurance and disintegration time. The drug release was about 98.5 % in 300 seconds(5 minutes). Hence, from
the present investigation it can be concluded that oral thin film formulation can be a potential novel drug
dosage form.
Key Words: oral thin film, Montelukast sodium, disintegration time

A simple, rapid, precise, sensitive and reproducible reverse phase high performance liquid chromatography
(RP-HPLC) method has been developed for the quantitative analysis of Thioguanine in pharmaceutical dosage
form. Chromatographic separation of Thioguanine was achieved on Waters Alliance-e2695, by using Waters
X- Bridge RP18, 150mm x 4.6mm, 3.5μm, column and the mobile phase containing 2.0gm Hexane-1-
Sulphonic acid is dissolved in 1lt water adjust pH-2.5 with OPA & ACN in the ratio of 40:60% v/v. The flow
rate was 1.0 ml/min; detection was carried out by absorption at 286nm using a photodiode array detector at
ambient temperature. The number of theoretical plates and tailing factor for Thioguanine was NLT 2000 and
should not more than 2 respectively. %Relative standard deviation of peak areas of all measurements always
less than 2.0. The proposed method was validated according to ICH guidelines. The method was found to be
simple, economical, suitable, precise, accurate & robust method for quantitative analysis of Thioguanine and
study of its stability.
Key words: HPLC, Thioguanine

Aim is to study the antidepressant activity of aqueous leaf extract of Hibiscus hirtus Linn in male Sprague
dawley rats. The Preliminary Phytochemical Analysis of aqueous extract of leaves of Hibiscus hirtus Linn
showed the presence of carbohydrates, sugars, proteins, aminoacids, flavanoids, triterpenoids, steroids, tannins,
phenols, fixed oils and fats and absence of alkaloid, glycosides, saponins, gums and mucilages. The present
study revealed the significant anti-depressant effect of aqueous extract of Hibiscus hirtus Linn leaves in
experimentally induced depression by Forced swim test and Tail suspension test models. The aqueous extract of
Hibiscus hirtus Linn leaves significantly decreased the immobility time in dose dependent manner which is an
indicator of antidepressant activity.
Key Words: Hibiscus hirtus Linn, antidepressant activity

Aim of the study is to evaluate the antidiabetic activity of the Hesperdin of Citrus aurantium (family: Rutaceae)
in experimatel animal models. The administration of streptozotocin produced a rise in the levels of glucose
compared to control group(p<0.01). Hesperidin treatment produced dose dependent decrease in the levels of
glucose compared with the streptozotocin treated group(p<0.01). These above data may indicate that the
protective effect of hesperdin against renal damage in diabetic rats.
Key Words: Antidiabetic activity, Hesperidin, Citrus aurantium

In the present study, the gelatin microspheres were formulated in such a way that it could control the drug
release based on disease conditions or by using external stimuli. The Celecoxib loaded gelatin
microspheres were formulated for intra- articular injection, thereby localizing the drug in the arthritic
knee. All formulated batches were free from residual glutaraldehyde and the method by which the
microspheres were formulated was completely eliminated the glutaraldehyde residue. Sodium
metabisulphite was used to terminate glutaraldehyde cross-linking and to remove unreacted
glutaraldehyde. In conclusion, the gelatin microspheres loaded with Celecoxib showed promising results
in reducing joint swelling in arthritic knee without drug induced toxicity. The results obtained from this
study indicated the scope of gelatin microspheres in the effective treatment of arthritis.
Key Words: Celecoxib, gelatin microspheres, arthritis.

Floating matrix tablet of cephalosporin antibacterial drug cefuroxime axetil can be formulated as an approach to
increase gastric residence time and thereby improve its bioavailability. Formulation F2, F6 gave bettercontrolled
drug release in comparison to the other formulations. The drug release pattern from the optimized
formulations was best fitted to Korsmeyer-Peppas model and zero order kinetics. Drug – excipients interaction
of optimized formulations was carried out by using FT-IR studies. In this analysis drug – excipients
compatibility interactions were not observed.
Key Words: cefuroxime axetil, Floating matrix tablet