To investigate the Nephroprotective activity of ethanol extract of Ficus dalhousiae on Gentamicin
induced nephrotoxicity in male Wistar rats. In this model of nephrotoxicity, 30 adult male wistar rats (150-
200gms) were evenly divided into 5 groups. Group-1 and Group-2 served as untreated and model controls
respectively, while Group-3, 4 and 5 were the treatments groups which were simultaneously treated with
standard, 200 and 400 mg/kg extract respectively, after each dose gentamicin (80 mg/kg, i.p.) for 10 day .
On 11
th
day, blood samples for biochemical parameters, while the rats kidneys for histology were obtained
under inhaled diether anaesthesia. Gentamicin treatment caused nephrotoxicity as evidenced by marked
elevation in blood urea, uric acid and creatinine. Co-administration of extract with Paracetanmol decreased
rise in blood urea, uric acid and creatinine. Apart from these, histopathological changes also showed the
protective nature of extract against Gentamicin induced necrotic damage of renal tissues. It was observed
that the ethanol extract of conferred nephroprotective activity by histopathological and biochemical
observation against Gentamicin induced nephrotoxicity in rats. In the near future could constitute a lead to
discovery of a novel drug for treatment of drug induced nephrotoxicity.
Key words: Ficus dalhousiae, Gentamicin, nephrotoxicity
KEY WORDS: Naltrexone, oxycodone, bulk dosage form

A simple and selective LC method is described for the determination of Escitalopram oxalate and Etizolam in
tablet dosage forms. Chromatographic separation was achieved on a c18 column using mobile phase consisting of a
mixture of 30 volumes of ammonium acetate buffer, 40 volumes of acetonitrile and 30 volumes of Methanol with
detection of 238 nm. Linearity was observed in the range 60-140 μg/ml for Escitalopram oxalate (r2 =0.999) and
6-14 μg /ml for Etizolam (r2 =0.996) for the amount of drugs estimated by the proposed methods was in good
agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed
by recovery studies at three different levels. Recovery experiments indicated the absence of interference from
commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the
repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be
used for routine analysis of pharmaceutical dosage form.
KEY WORDS: Liquid chromatography (LC), RSD Relative standard deviation, r2 correlation coefficient.

To investigate the hepato and nephroprotective activity of ethanol extract of Sebastiana
chamaelea on Paracetamol induced nephrotoxicity in male Wistar rats. In this model of nephrotoxicity,
30 adult male wistar rats (150-200gms) were evenly divided into 5 groups. Group-1 and Group-2
served as untreated and model controls respectively, while Group-3, 4 and 5 were the treatments groups
which were simultaneously treated with standard, 2 0 0 and 400 mg/kg e x t r a c t respectively, after
each dose of Paracetamol (200 mg/kg, i.p. for 3 days) from4 to 14 days . On 11
th
day, blood samples for
biochemical parameters, while the rats kidneys for histology were obtained under inhaled diether
anaesthesia. Paracetamol treatment caused hepato a n d nephrotoxicity as evidenced by marked
elevation in blood urea, uric acid and creatinine, bilirubin. Co-administration of e x t r a c t with
Paracetanmol decreased rise in blood urea, uric acid and creatinine, bilirubin. Apart from these,
histopathological changes also showed the protective nature of extract against Paracetamol induced
necrotic and hepatic damage of renal and hepatic tissues. It was observed that the ethanol extract of
conferred nephroprotective and hepatoprotective activities by histopathological and biochemical
observation against Paracetamol induced nephrotoxicity and hepatotoxicity in rats. In the near future could
constitute a lead to discovery of a novel drug for treatment of drug induced nephrotoxicity and hepato
toxicity.
KEY WORDS: Sebastiana chamaelea, Paracetamol induced nephrotoxicity, hepato and nephroprotective activity

ABSTRACT
“Utricularia reticulata” is a medium to large sized probably annual carnivorous plant which is used by the
tribes for the treatments of ulcers, wound healing, and neutralizing venoms of snakes, spiders, eye diseases. The
study highlights the antibacterial studies of the plant thereby increasing the utilization of this commonly
available plant for its medicinal property. The whole plants were collected, dried and subjected to successive
solvent extraction. The phtyochemical and pharmacological studies were carried out. Further the extracts were
tried for antioxidant activity and antimicrobial studies. Ethyl acetate extracts were shown significant antioxidant
and antibacterial properties. Inspite of the data reveled form phytochemical screening and literature survey the
phytoconstituents like alkaloids, glycosides, phenolic and flavonoids, flavonones, terpenoids and sterols present
in the plant were responsible for the activities.
Keyword: Utricularia reticulata, antioxidant, antibacterial.

ABSTRACT
The purpose of this research project was to prepare fast disintegrating tablets of Diclofenac sodium, an NSAID
drug by direct compression method using natural superdisintegrant (treated banana powder) and evaluate their
various in-vitro properties. Diclofenac sodium was chosen as the drug with an aim to develop fast
disintegrating tablets by direct compression method, which disintegrates quickly and have a release of drug
faster than conventional tablets. A natural superdisintegrant (treated banana powder) with different
concentrations were used in the development of formulations by direct compression method. The prepared
tablets were evaluated for pre and post compression parameters like angle of repose, bulk density, tapped
density, drug content, wetting time, in-vitro disintegration time, in-vitro dissolution studies.
KEY WORDS: Diclofenac sodium, treated banana powder, superdisintegrant, fast disintegrating tablet

ABSTRACT
Chassalia curviflora is an evergreen shrub grows in tropical region. It is endemic to East Asia. It has potential
uses like anti hypertensive, anti bacterial and traditionally used for eye infection, ear infection and insect bites.
The present study highlights the phytochemical and invitro pharmacological studies of various extracts of
Chassalia curviflora. The phytochemical screening of the plant extracts shows the presence of various chemical
constituents. The chloroform extract shows potent antioxidant and anti-inflammatory activity when compared to
other extracts. The presence of chemical constituents such as alkaloids, phenolics, flavanoids and saponins may
be responsible for the anti oxidant and anti-inflammatory activities.
KEYWORDS: Chassalia curviflora, antioxidant, anti inflammatory.

ABSTRACT
A novel series of six different derivatives of some NSAID chalcones were synthesized and characterized by
physical & spectral interpretations like m.p., FT-IR & H1 NMR are confirmed. The derivatives of some NSAID
chalcones were prepared from chalcone derivatives and hydrazine. The initial compounds for synthesizing
derivatives are paracetamol ,aspirin and different aldehydes. The synthesized compounds are useful as a lead
compounds for various diseases like antiulcer, antitumour, herbicidal, antifungal activites some infectious
diseases. The derived chalcone pyrazoline derivatives were characterized by thin layer chromatography, infrared
spectroscopic and NMR methods. The biological activity was tested and found to have anti-microbial
activity against bacteria like E.coli (Gram negative organism), Pseudomonas aeuroginosa (Gram negative
organism), Staphylococcus aureus (Gram positive organism) and Fungal Organism of Sacchromyces Species,
Aspergillus Niger, Candida Albicans using filter-paper disc method. Most of the compounds exhibit mild to
moderate anti bacterial activity as well as anti fungal activity against all the microbes tested.
Key Words: NSAID chalcones, insilico prediction, antimicrobial activity

ABSTRACT
Aim of the study is to synthesize some new 2, 4, 6-trisubstituted pyrimidines by conventional method. All the
synthesized compounds were characterized by FT-IR and1H-NMR spectral studies and the structures were
established. All the synthesized compounds were predicted for biological properties by using PASS computer
programme and screened for Anti-Bacterial activity in Staphylococcus aureus&E.coliby using Ciprofloxacin as
standard. From the results of Anti-bacterial activity, it was clear that the compounds SB-4, SB-5, SB-8 & SB-9
exhibit significant activity in comparison with that of standard. This may be due to the presence of electron
donating groups like Nitro group at position -6 of the parent molecule.
Key Words: 2,4,6-Trisubstituted Pyrimidines, anti-bacterial activity

ABSTRACT
The present work is aimed to formulate spherical agglomerates of telmisartan by crystallo-co-agglomeration
technique and enhance the micromeritic properties, solubility and dissolution rate of telmisartan, a poorly
water soluble anti-hypertensive drug. Chloroform-water system containing PEG 6000 & HPMC in different
concentration was used as the crystallization medium. Chloroform act as good solvent and bridging liquid for
telmisartan and water as bad solvent. The agglomerates were characterized to various physicochemical
evaluations such as practical yield, drug content, particle size, FTIR spectroscopy, scanning electron
microscopy, micromeritic properties and solubility analysis. The dissolution studies were carried out using
PBS of pH 7.4 as dissolution media maintained at 37.5°C for 70 minutes. The dissolution data demonstrate that
the rate of drug release is dependent upon the nature and concentration of polymer used in the formulation.
Formulation TP2 (combination of telmisartan and PEG 6000) in the ratio 1:1.5 was selected as an optimum
formulation which showed better results with respect to drug release, solubility and micromeritics when
compared to other formulation.
Key Words: Telmisartan, Crystallo-co-agglomerates, PEG6000, HPMC.

ABSTRACT
Substituted 2-methylamino benzimidazoles were synthesized by refluxing 2-methylamino benzimidazole with
urea and thiourea in presence of methanol. Synthesis of 2-methylamino benzimidazole from o-phenylene
diamine by refluxing with glycine in presence of HCl. The structures of final synthesized compounds were
assigned on the basis of IR spectral data. All the newly synthesized compounds were screened for their in-vitro
antimicrobial activity against various species of bacteria by cup plate method. The zone of inhibition exhibited
by all the derivatives at a concentration of 250μg and 500μg was compared to that of standard ciprofloxacin at a
concentration of 5μg. The compounds were good antibacterial agents against the tested organisms when
compared to the standard ciprofloxacin.
Key Words: Benzimadazole, o-phenylene diamine, glycine, antimicrobial.

ABSTRACT
5,7-dihydroxy-2[2-(3-methylbut-2-en-1-yl)phenyl]-2,3-dihydro-4H-1-benzopyran-4-onewere prepared with
different chloro phenyl acetic acid under mild conditions to give corresponding prenylated flavones, the title
compounds in good yields. Lead compound synthesized were characterized by physical (Rf values, Melting
point, Molecular weight, Molecular formula) and spectral data (1H NMR, IR, Mass spectra). In carageenan
induced paw edema compounds 4,7,10 significantly inhibited the edema in a dose dependent manner. The paw
volume in rats pretreated with lower dose of compounds 4,7,10 (100 mg/kg/day), higher dose of compounds
4,7,10 (200 mg/kg/day) and indomethacin (10 mg/kg/day) at 2nd hr were found to be 0.191  0.0061 ml, 0.158 
0.0042** ml and 0.1369  0.0054** ml.
Key Words: prenylated 5,7-dihydroxy flavonoid, anti inflammatory activity.

A study was carried out to investigate the efficiency of different natural binding agent such as gum of Okra fruit,
gum of Hibiscus flower, gummy exudates of Moringa tree and gum of Aloe-Vera in Moringa oleifera tablet.
Moringa oleifera is widely used for both nutrition and medicinal activity. The physical parameters used were
weight variation, harness, friability, disintegration time and antimicrobial assay. The results of this study showed
that Aloe-Vera gum produced tablet with higher hardness, disintegration time and increased antimicrobial
activity. In conclusion, Aloe-Vera gum has good tablet binding properties and could be employed as a substitute
for more expensive binders in fast or immediate release tablet.

The roots of the plant Chassalia curviflora (Rubiaceae) have been used by Kani tribes for the treatment of
colic pain and to heal wounds and pimples. Oil boiled with the juice of the leaves is used for eye and ear
disease, ulcer and sore throat. Decoction of root is given as a remedy in phlegm, rheumatism and pneumonia.
The work highlights pharmacognostic studies and evaluation of antioxidant, anti-inflammatory activity of
various extracts of the roots of plant Chassalia curviflora. The pharmacognostic studies like loss on drying,
ash values total ash value, extractive values, crude fibre content were done on the plant proved to be a tool for
the authentication and identification of the plant The antioxidant activity studies using Iron chelating assay,
DPPH radical scavenging assay and total antioxidant assay showed that ethyl acetate extract could be a
promising source of natural antioxidants. The anti inflammatory activities by inhibition of protein denaturation
and proteinase inhibitory assay showed ethyl acetate extract to possess potent anti inflammatory activity. Thus
the roots of the plant Chassalia curviflora can be suggested to be used in diseases like phlegm, rheumatism
and pneumonia due to its antioxidant and anti inflammatory properties.
Key words: Chassalia curviflora, antioxidant, anti inflammatory

Antimicrobial agents play key role in the management of infections. Challenges faced by the chemists to
develop a wide spectrum antimicrobial agent were the antibiotic resistance occurred due to the misuse of these
agents. Pyrimidine is prevalent in numerous natural products and posses a wide range of biological activities.
The work includes the synthesis of some 1,3-dihydro pyrimidine derivatives from different substituted
aldehydes. Pyrimindine derivatives (6a,6b,6d) were synthesized from different aromatic aldehydes and
screened for their antimicrobial properyies in gram positive (Bacillus subtitis, Staphylococous aureus) and gram
negative (Pseudomonas aeruginosa, Escherichia coli) organisms at concentration 400μg/disc and 200μg/disc.
The compound 6b (phenyl derivative) have significant antimicrobial properties than other synthesized
derivatives.
Key words: Purines, Pyrimidines, substituted aldehydes, antibacterial.

ABSTRACT
Worldwide cancer is the major cause of death. Millions of people died due to different types of cancer such as
lung cancer, liver cancer, breast cancer, stomach cancer, blood cancer etc. Couples of therapies like
chemotherapy, radiation, surgery and targeted therapy are available with some detrimental effect. Natural
phytochemicals are prominent strategy for prevention, treating, and curing cancer. There are many plants
(phytochemicals) possess anti-cancer activity that exhibits additive or synergistic activity. Generally these
phytochemicals treat cancer by different mechanisms like augmenting apoptosis, cell cycle arrest, targeting to
some specific cancer inducing proteins, increasing cytotoxicity etc. In this study a methanol extractfrom whole
plant Nymphoides krishnakesara Var. Bispinosa male and female plantswere evaluated for in vitro anticancer
activity by MTT cell viability assay and in vivo anticancer activity by DAL (Dalton’s Ascites Lymphoma)
Induced Anticancer Study.The viability of cells were evaluated byMTT assay method.These studies indicates
positive anticancer activity of the extractNymphoides krishnakesara Var. Bispinosa male and female (whole
plant) by invitro as well as in vivo study.
Key Words: MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide), DAL (Dalton’s Ascites
Lymphoma), synergistic, anticancer.